Simple determination of high urinary excretion of 5-hydroxyindole-3-acetic acid with ferric chloride.

Automated on-line solid-phase extraction coupled with HPLC for measurement of 5-hydroxyindole-3-acetic acid in urine.

BACKGROUND Quantification of 5-hydroxyindole-3-acetic acid (5-HIAA) in urine is useful in diagnosing and monitoring of patients with carcinoid tumors and in the study of serotonin (5-hydroxytryptamine) metabolism in various disorders. We describe an automated method that incorporates on-line solid-phase extraction (SPE) and HPLC to measure urinary 5-HIAA. METHODS Automated prepurification of ...

Simultaneous determination of serotonin and 5-hydroxyindole-3-acetic acid in human urine by automated precolumn derivatization and semi-microbore column liquid chromatography with fluorescence detection.

An improved high-performance liquid-chromatographic (HPLC) assay for the simultaneous determination of serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) in human urine is reported. Following the automated precolumn derivatization of urinary 5-HT and 5-HIAA with benzylamine, the derivatives are separated by isocratic elution on a reversed-phase C18 semi-microbore column, and are fluoro...

Association between urinary albumin excretion and plasma 5-hydroxyindole-3-acetic acid concentration in men with type 2 diabetes.

S erotonin (5-hydroxytryptamine [5-HT]) mediates vasoconstriction and induces the activation of platelets, which may promote atherosclerosis. Plasma 5-HT concentrations have been reported to be high in diabetic patients (1,2); this may be one of the underlying mechanisms of diabetes complications. The 5-HT2A receptor has been identified in glomerular mesangial cells (3), suggesting involvement ...

Association between urinary albumin excretion and plasma-hydroxyindole-3-acetic acid concentration in men with type 2 diabetes

Colorimetric vs liquid-chromatographic determination of urinary 5-hydroxyindole-3-acetic acid.

The popular colorimetric assay for urinary 5-hydroxyindole-3-acetic acid based on the reaction with 1-nitroso-2-naphthol has been criticized for lack of specificity. Goldenberg (Clin. Chem. 19: 38-44, 1973) proposed a modification of this method, in which 2-mercaptoethanol is used to discharge unwanted chromophores. We compared results by both the unmodified and modified colorimetric methods wi...